Application
Immunohistochemistry staining of MLF1IP in prostate tissue using MLF1IP Antibody.| Product Name | MLF1IP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLF1IP, CENP-U, CENP50, CENPU50, Centromere protein U, ICEN24, KLIP1, Polo-box-interacting protein 1, MLF1-interacting protein, CENP-50, CENPU, Centromere protein of 50 kDa, MLF1 interacting protein, PBIP1 |
| Product description | Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis. |
| Immunogen | MLF1IP antibody was raised against amino acids surrounding Thr78 of MLF1IP (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation: |
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| Species Reactivity | BovineChimpanzeeDogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MLF1IP |
|---|---|
| Alternative Names | MLF1IP CENP-U CENP50 CENPU50 Centromere protein U ICEN24 KLIP1 Polo-box-interacting protein 1 MLF1-interacting protein CENP-50 CENPU Centromere protein of 50 kDa MLF1 interacting protein PBIP1 |
| Tissue Specificity | pThr78 |
Application
Immunohistochemistry staining of MLF1IP in prostate tissue using MLF1IP Antibody.| Application Notes | MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store MLF1IP antibody at -20 ˚C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 ˚C or below. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
