Application
Immunohistochemistry of MLIP in human heart tissue with MLIP antibody at 5 μg/mL.Application
Immunofluorescence of MLIP in human heart tissue with MLIP antibody at 20 μg/mL.Application
Western blot analysis of MLIP in 293 cell lysate with MLIP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | MLIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLIP Antibody: C6orf142, C6orf142, Muscular LMNA-interacting protein, Muscular-enriched A-type laminin-interacting protein |
| Product description | MLIP Antibody: The muscle-enriched A-type lamin-interacting protein (MLIP) is ubiquitously expressed, although most predominantly in heart, skeletal and smooth muscle, and interacts directly and co-localizes with lamin A and C in the nuclear envelope. MLIP also co-localizes with promyelocytic leukemia (PML) bodies within the nucleus. Decreasing lamin A/C expression with siRNA results in the upregulation and mislocalization of MLIP, suggesting that MLIP localization to the nuclear envelope is dependent on the lamin proteins. It has been suggested that MLIP may function as a tethering protein linking LMNA to factors within the nuclear envelope of within PML bodies1) Ahmady E, Deeke SA, Rabaa S, et al. Identification of a novel muscle A-type lamin-interacting protein (MLIP). J. Biol. Chem. 2011; 286:19702-13. |
| Immunogen | MLIP antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human MLIP. The immunogen is located within the last 50 amino acids of MLIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MLIP antibody can be used for detection of MLIP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MLIP |
|---|---|
| Alternative Names | MLIP Antibody: C6orf142 C6orf142 Muscular LMNA-interacting protein Muscular-enriched A-type laminin-interacting protein |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | At least three isoforms of MLIP are known to exist. |
Application
Immunohistochemistry of MLIP in human heart tissue with MLIP antibody at 5 μg/mL.
Application
Immunofluorescence of MLIP in human heart tissue with MLIP antibody at 20 μg/mL.
Application
Western blot analysis of MLIP in 293 cell lysate with MLIP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | MLIP antibody can be used for detection of MLIP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
