Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | MLK1/2 (phospho Thr312/266) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M3K9, PRKE1, mixed-lineage protein kinase 1 |
| Product description | The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.1) Durkin J.T., Biochemistry 43:16348-16355(2004). |
| Immunogen | MLK1/2 (phospho Thr312/266) antibody was raised against a peptide sequence around phosphorylation site of threonine 312/266 (A-G-T (p)-Y-A) derived from Human MLK1/2. |
| Modification | p-Thr312/266 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K9, MAP3K10 |
|---|---|
| Alternative Names | M3K9 PRKE1 mixed-lineage protein kinase 1 |
| Molecular Weight(MW) | 121 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266. |
| Entrez Gene | 4293/4294 |
|---|---|
| Protein Accession | NP_001271159.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚,C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
