Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody at 1:1000 at each lane.| Product Name | MLK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase kinase kinase MLK4, Mixed lineage kinase 4, MLK4 {ECO:0000303|Ref1} |
| Product description | Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the p38 and JNK signaling cascades. Systematic mutational analyses of tyrosine kinases reveal that a minimum of 30% of colorectal cancer contain at least one mutation in the tyrosine kinases; sampling of colorect cancer cells suggest an important role of MLK4 in this process.1) Gregory,S.G., Nature 441 (7091), 315-321 (2006) |
| Immunogen | This MLK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human MLK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MLK4 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase MLK4 Mixed lineage kinase 4 MLK4 {ECO:0000303|Ref1} |
| Molecular Weight(MW) | 114 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysates from K562, Ramos cell line (from left to right), using MLK4 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
