Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:500 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of MMP13 using rabbit polyclonal MMP13 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2).| Product Name | MMP13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Collagenase 3, 3424-, Matrix metalloproteinase-13, MMP-13, MMP13 |
| Product description | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.1) Roy-Beaudry, M., et al., Arthritis Rheum. 48(10):2855-2864 (2003). |
| Immunogen | This MMP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MMP13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MMP13 |
|---|---|
| Alternative Names | Collagenase 3 3424- Matrix metalloproteinase-13 MMP-13 MMP13 |
| Molecular Weight(MW) | 54 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MMP13 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:500 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of MMP13 using rabbit polyclonal MMP13 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP13 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
