Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.1) Nozell, S., et al., (2004) J. Biol. Chem. 279 (37), 38577-38589.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MMP9.
Antibody used in IHC on Mouse prostate cancer at 10 ug/ml.
Application
Antibody used in WB on Human K562 at 1.25 ug/ml.
Positive Control
1) Cat. No. 1204 - K562 Cell Lysate
Application Notes
WB:1:500~1:2000 IHC:1:50~1:200
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store MMP9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt