Application
Mouse Akt1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Product Name | Akt1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAC-alpha serine/threonine-protein kinase, AKT1 kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, Thymoma viral proto-oncogene, Akt1, Akt, Rac |
| Product description | This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Chang, Z., et al. Dev. Biol. 347(2):384-391(2010) |
| Immunogen | This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Akt1 |
|---|---|
| Alternative Names | RAC-alpha serine/threonine-protein kinase AKT1 kinase Protein kinase B PKB Protein kinase B alpha PKB alpha Proto-oncogene c-Akt RAC-PK-alpha Thymoma viral proto-oncogene Akt1 Akt Rac |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Human |
Application
Mouse Akt1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
