Application
Mouse Akt2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).| Product Name | Akt2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, Akt2 |
| Product description | General protein kinase capable of phosphorylating several known proteins.1) Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010) Mouton, V., et al. Biochem. J. 431(2):267-275(2010) Lazorchak, A.S., et al. Mol. Cell 39(3):433-443(2010) Goncalves, M.D., et al. PLoS ONE(9), E12707 (2010)Rotte, A., et al. Cell. Physiol. Biochem. 25(6):695-704(2010) |
| Immunogen | This Mouse Akt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of mouse Akt2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Akt2 |
|---|---|
| Alternative Names | RAC-beta serine/threonine-protein kinase Protein kinase Akt-2 Protein kinase B beta PKB beta RAC-PK-beta Akt2 |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Mouse Akt2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
