Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | Bckdk Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN, Bckdk |
| Product description | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).1) Pagliarini, D.J., et al. Cell 134(1):112-123(2008) |
| Immunogen | This Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of mouse Bckdk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Bckdk |
|---|---|
| Alternative Names | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial Branched-chain alpha-ketoacid dehydrogenase kinase BCKD-kinase BCKDHKIN Bckdk |
| Molecular Weight(MW) | 47 kDa |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM285025