Application
mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
mouse BID Antibody (S61)IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry.Application
Western blot analysis of (S61) in mouse spleen tissue lysates (35ug/lane)| Product Name | BID Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid |
| Product description | Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.1) Ziporen,L., J. Immunol. 182 (1), 515-521 (2009) |
| Immunogen | This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from mouse BID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Bid |
|---|---|
| Alternative Names | BH3-interacting domain death agonist p22 BID BID BH3-interacting domain death agonist p15 p15 BID BH3-interacting domain death agonist p13 p13 BID BH3-interacting domain death agonist p11 p11 BID Bid |
| Molecular Weight(MW) | 22 kDa |
Application
mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
mouse BID Antibody (S61)IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry.
Application
Western blot analysis of (S61) in mouse spleen tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
