Western blot analysis of lysates from 293, mouse NIH/3T3, rat C6 cell line and mouse kidney tissue lysate(from left to right), using Mouse Cdk9 Antibody at 1:1000 at each lane.
Cyclin-dependent kinase 9, Cell division protein kinase 9, Cdk9
Product description
Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro (By similarity).1) Yokoyama, S., et al. Dev. Cell 17(6):836-848(2009)
Immunogen
This Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-278 amino acids from the C-terminal region of mouse Cdk9.
Western blot analysis of lysates from 293, mouse NIH/3T3, rat C6 cell line and mouse kidney tissue lysate(from left to right), using Mouse Cdk9 Antibody at 1:1000 at each lane.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.