Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).Application
Mouse Chuk Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | Chuk Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B inhibitor kinase alpha, NFKBIKA, Ch |
| Product description | Chuk acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. |
| Immunogen | This Mouse Chuk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-578 amino acids from the C-terminal region of mouse Chuk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Chuk |
|---|---|
| Alternative Names | Inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase alpha IKK-A IKK-alpha IkBKA IkappaB kinase Conserved helix-loop-helix ubiquitous kinase I-kappa-B kinase 1 IKK1 Nuclear factor NF-kappa-B inhibitor kinase alpha NFKBIKA Ch |
| Molecular Weight(MW) | 85 kDa |
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).
Application
Mouse Chuk Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
