Application
Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Dnmt2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog MmuIIP, DNA MTase homolog MmuIIP, MMmuIIP, Met-2, Trdmt1, Dnmt2, Met2 |
| Product description | mDnmt2 does not appear to be active as a DNA methyltransferase; however, its strong binding to DNA suggests that it may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. mDnmt2 is strongly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. This protein belongs to the 5-cystosine methyltransferase family.1) Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998). |
| Immunogen | This Mouse Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the N-terminal region of mouse Dnmt2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | Trdmt1 |
|---|---|
| Alternative Names | tRNA (cytosine(38)-C(5))-methyltransferase DNA (cytosine-5)-methyltransferase-like protein 2 Dnmt2 DNA methyltransferase homolog MmuIIP DNA MTase homolog MmuIIP MMmuIIP Met-2 Trdmt1 Dnmt2 Met2 |
| Molecular Weight(MW) | 47 kDa |
Application
Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
