Application
Western Blot at 1:1000 dilution Lane 1: mouse liver lysate Lane 2: mouse thymus lysate Lysates/proteins at 20 ug per lane.| Product Name | Ern1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, Ern1 {ECO:0000312|MGI:MGI:1930134} |
| Product description | Ern1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.1) Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16113-16118(2010) |
| Immunogen | This Mouse Ern1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 827-855 amino acids from the C-terminal region of mouse Ern1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ern1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase/endoribonuclease IRE1 Endoplasmic reticulum-to-nucleus signaling 1 Inositol-requiring protein 1 Ire1-alpha IRE1a Serine/threonine-protein kinase Endoribonuclease 3126- Ern1 {ECO:0000312|MGI:MGI:1930134} |
| Molecular Weight(MW) | 110 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: mouse liver lysate Lane 2: mouse thymus lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
