Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Irak1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-1 receptor-associated kinase 1, IRAK, IRAK-1, Pelle-like protein kinase, mPLK, Irak1, Il1rak |
| Product description | Irak1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The kinase activity of this enzyme may not be required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.1) Vaughan, T., et al. Mol. Immunol. 47(15):2515-2518(2010) |
| Immunogen | This Mouse Irak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-433 amino acids from the Central region of mouse Irak1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Irak1 |
|---|---|
| Alternative Names | Interleukin-1 receptor-associated kinase 1 IRAK IRAK-1 Pelle-like protein kinase mPLK Irak1 Il1rak |
| Molecular Weight(MW) | 77 kDa |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
