Application
Mouse Map3k5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).Application
Western blot analysis of Map3k5 using rabbit polyclonal Mouse Map3k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.WB
(A) Western blotting analysis of MITF, ERK, p-ERK, Dicer, MAP3K5 and CREB protein expression in the skin of CDK5-knockdown and wild-type mice.| Product Name | Map3k5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death (By similarity). |
| Immunogen | This Mouse Map3k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1175-1203 amino acids from the C-terminal region of mouse Map3k5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Map3k5 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase 5 Apoptosis signal-regulating kinase 1 ASK-1 MAPK/ERK kinase kinase 5 MEK kinase 5 MEKK 5 Map3k5 Ask1 Mekk5 |
| Molecular Weight(MW) | 155 kDa |
Application
Mouse Map3k5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Application
Western blot analysis of Map3k5 using rabbit polyclonal Mouse Map3k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.
WB
(A) Western blotting analysis of MITF, ERK, p-ERK, Dicer, MAP3K5 and CREB protein expression in the skin of CDK5-knockdown and wild-type mice.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
