Application
Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody at 1:1000 at each lane.Application
Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Overlay histogram showing A431 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat| Product Name | Pbk Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphokine-activated killer T-cell-originated protein kinase, PDZ-binding kinase, T-LAK cell-originated protein kinase, Pbk, Topk |
| Product description | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity).1) Zykova, T.A., et al. Clin. Cancer Res. 12(23):6884-6893(2006) |
| Immunogen | This Mouse Pbk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of mouse Pbk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pbk |
|---|---|
| Alternative Names | Lymphokine-activated killer T-cell-originated protein kinase PDZ-binding kinase T-LAK cell-originated protein kinase Pbk Topk |
| Molecular Weight(MW) | 37 kDa |
Application
Western blot analysis of lysate from mouse testis tissue lysate, using Mouse Pbk Antibody at 1:1000 at each lane.
Application
Western blot analysis of lysate from Hela cell line, using Mouse Pbk Antibody at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded R. testis section using Mouse Pbk Antibody(N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Overlay histogram showing A431 cells stained with Antibody (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor 488 goat| Application Notes | For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
