Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).Application
Western blot analysis of Pkmyt1 using rabbit polyclonal Mouse Pkmyt1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Product Name | Pkmyt1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, Pkmyt1, Myt1 |
| Product description | Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity). |
| Immunogen | This Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the N-terminal region of mouse Pkmyt1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pkmyt1 |
|---|---|
| Alternative Names | Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase Myt1 kinase Pkmyt1 Myt1 |
| Molecular Weight(MW) | 54 kDa |
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).
Application
Western blot analysis of Pkmyt1 using rabbit polyclonal Mouse Pkmyt1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
