Application
Mouse Prkaca/Prkacb Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkaca/Prkacb Antibody for immunohistochemistry.Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).| Product Name | Prkaca/Prkacb Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, Prkacb, Pkacb |
| Product description | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.1) Ribeiro, R.A., et al. Br. J. Nutr. 104(8):1148-1155(2010) |
| Immunogen | This Mouse Prkaca/Prkacb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-319 amino acids from the C-terminal region of mouse Prkaca/Prkacb. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Prkacb |
|---|---|
| Alternative Names | cAMP-dependent protein kinase catalytic subunit beta PKA C-beta Prkacb Pkacb |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Mouse Prkaca/Prkacb Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkaca/Prkacb Antibody for immunohistochemistry.
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Prkaca/Prkacb antibody detected the Prkaca/Prkacb protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
