Application
Western blot analysis in mouse spleen tissue lysates (35ug/lane).| Product Name | Prr5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proline-rich protein 5, Protein observed with Rictor-1, Protor-1, Prr5 {ECO:0000250|UniProtKB:P85299}, Protor1 |
| Product description | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer (By similarity).1) Johnstone, C.N., et al. Genomics 85(3):338-351(2005) |
| Immunogen | This Mouse Prr5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of mouse Prr5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Prr5 |
|---|---|
| Alternative Names | Proline-rich protein 5 Protein observed with Rictor-1 Protor-1 Prr5 {ECO:0000250|UniProtKB:P85299} Protor1 |
| Molecular Weight(MW) | 43 kDa |
Application
Western blot analysis in mouse spleen tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
