Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | Rnasen Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ribonuclease 3, Protein Drosha, Ribonuclease III, RNase III, Drosha, Etohi2, Rn3, Rnasen |
| Product description | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).1) Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010) |
| Immunogen | This Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Drosha |
|---|---|
| Alternative Names | Ribonuclease 3 Protein Drosha Ribonuclease III RNase III Drosha Etohi2 Rn3 Rnasen |
| Molecular Weight(MW) | 159 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
