Application
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | Shc1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SHC-transforming protein 1, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1, SH2 domain protein C1, Shc1, Shc, ShcA |
| Product description | Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.1) Ursini-Siegel, J., et al. Cancer Res. 70(20):7776-7787(2010) |
| Immunogen | This Mouse Shc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-405 amino acids from the Central region of mouse Shc1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Shc1 |
|---|---|
| Alternative Names | SHC-transforming protein 1 SHC-transforming protein A Src homology 2 domain-containing-transforming protein C1 SH2 domain protein C1 Shc1 Shc ShcA |
| Molecular Weight(MW) | 63 kDa |
Application
Western Blot at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
