Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).Application
Western blot analysis of lysate from NCI-H1299 cell line, using Mouse Stk11 Antibody at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Product Name | Stk11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase STK11, Liver kinase B1 homolog, LKB1, mLKB1, Stk11 {ECO:0000312|MGI:MGI:1341870} |
| Product description | Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases (By similarity). Tumor suppressor.1) Gao, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18892-18897(2010) |
| Immunogen | This Mouse Stk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-420 amino acids from the C-terminal region of mouse Stk11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:500-1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Stk11 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase STK11 Liver kinase B1 homolog LKB1 mLKB1 Stk11 {ECO:0000312|MGI:MGI:1341870} |
| Molecular Weight(MW) | 49 kDa |
Application
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Application
Western blot analysis of lysate from NCI-H1299 cell line, using Mouse Stk11 Antibody at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Application Notes | For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:500-1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
