Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).| Product Name | Xaf1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XIAP-associated factor 1, BIRC4-binding protein, Xaf1, Birc4bp, Xiapaf1 |
| Product description | Xaf1 seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).1) Bai, Y., et al. J. Biol. Chem. 283(11):6832-6842(2008) |
| Immunogen | This Mouse Xaf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of mouse Xaf1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Xaf1 |
|---|---|
| Alternative Names | XIAP-associated factor 1 BIRC4-binding protein Xaf1 Birc4bp Xiapaf1 |
| Molecular Weight(MW) | 31 kDa |
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
