Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
MPP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
Western blot analysis of MPP1 using rabbit polyclonal MPP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.| Product Name | MPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 55 kDa erythrocyte membrane protein, p55, Membrane protein, palmitoylated 1, MPP1, DXS552E, EMP55 |
| Product description | This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.1) Seo, P.S., et al. Exp. Biol. Med. (Maywood) 234(3):255-262(2009) |
| Immunogen | This MPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-327 amino acids from the Central region of human MPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPP1 |
|---|---|
| Alternative Names | 55 kDa erythrocyte membrane protein p55 Membrane protein palmitoylated 1 MPP1 DXS552E EMP55 |
| Molecular Weight(MW) | 52 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
MPP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
Western blot analysis of MPP1 using rabbit polyclonal MPP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPP1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
