Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MPP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3, MPP3, DLG3 |
| Product description | This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.1) Qin, L., et al. Zhonghua Gan Zang Bing Za Zhi 15(7):509-512(2007) |
| Immunogen | This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MPP3 |
|---|---|
| Alternative Names | MAGUK p55 subfamily member 3 Discs large homolog 3 Protein MPP3 MPP3 DLG3 |
| Molecular Weight(MW) | 66 kDa |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of MPP3 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
