Application
Immunocytochemistry of MPYS in A-20 cells with MPYS antibody at 5 μg/mL.Application
Immunofluorescence of MPYS in A-20 cells with MPYS antibody at 20 ug/mL.Application
Western blot analysis of MPYS in (A) K562 and (B) Jurkat cell lysate with MPYS antibody at 1 μg/mL.| Product Name | MPYS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING |
| Product description | MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.1) Jin L, Waterman PM, Jonscher KR, et al. MPYS, a novel membrane tetraspanner, is associated with major histocompatibility complex class II and mediates transduction of apoptotic signals. Mol. Cell. Biol.2008; 28:5014-26. |
| Immunogen | MPYS antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260 - 310 of MPYS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| MPYS antibody can be used for detection of MPYS by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM173 |
|---|---|
| Alternative Names | MPYS Antibody: ERIS MITA MPYS NET23 STING ERIS Stimulator of interferon genes protein Endoplasmic reticulum interferon stimulator hSTING |
| Molecular Weight(MW) | Predicted: 31, 42 kDa Observed: 32 kDa |
| Sequence Similarities | 4) Cat. No. 17-208 - A-20 Cell Slide |
Application
Immunocytochemistry of MPYS in A-20 cells with MPYS antibody at 5 μg/mL.
Application
Immunofluorescence of MPYS in A-20 cells with MPYS antibody at 20 ug/mL.
Application
Western blot analysis of MPYS in (A) K562 and (B) Jurkat cell lysate with MPYS antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | MPYS antibody can be used for detection of MPYS by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MPYS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MPYS Antibody is supplied in PBS containing 0.02% sodium azide. |
