Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated| Product Name | MRPL28 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1 |
| Product description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.1) Lamesch, P., et al. Genomics 89(3):307-315(2007) |
| Immunogen | This MRPL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the N-terminal region of human MRPL28. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MRPL28 |
|---|---|
| Alternative Names | 39S ribosomal protein L28 mitochondrial L28mt MRP-L28 Melanoma antigen p15 Melanoma-associated antigen recognized by T-lymphocytes MRPL28 MAAT1 |
| Molecular Weight(MW) | 30 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
