Application
MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry.Application
Western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).| Product Name | MRPS12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 28S ribosomal protein S12, mitochondrial, MRP-S12, S12mt, MT-RPS12, MRPS12, RPMS12, RPSM12 |
| Product description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.1) Zanotto, E., et al. Biochim. Biophys. Acta 1789(5):432-442(2009) |
| Immunogen | This MRPS12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-59 amino acids from the Central region of human MRPS12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MRPS12 |
|---|---|
| Alternative Names | 28S ribosomal protein S12 mitochondrial MRP-S12 S12mt MT-RPS12 MRPS12 RPMS12 RPSM12 |
| Molecular Weight(MW) | 15 kDa |
Application
MRPS12 Antibody (Center K43) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPS12 Antibody (Center K43) for immunohistochemistry.
Application
Western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
