Application
Immunohistochemical analysis of paraffin - embedded human rectum carcinoma tissue showing nuclear and cytoplasmic localization using MSH2 antibody with DAB staining.Application
Western blot analysis using MSH2 monoclonal antibody against A431 cell lysate.| Product Name | MSH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MutS protein homolog 2, hMSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1 |
| Product description | MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.1) Papadopoulos, N. 1994. Science 263: 1625-1629. |
| Immunogen | Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:200 - 1:1,000 IHC(F):Dilution 1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MSH2 |
|---|---|
| Alternative Names | MutS protein homolog 2 hMSH2 FCC1 COCA1 HNPCC LCFS2 HNPCC1 |
Application
Immunohistochemical analysis of paraffin - embedded human rectum carcinoma tissue showing nuclear and cytoplasmic localization using MSH2 antibody with DAB staining.
Application
Western blot analysis using MSH2 monoclonal antibody against A431 cell lysate.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Western Blot:Dilution 1:200 - 1:1,000 IHC(P):Dilution 1:200 - 1:1,000 IHC(F):Dilution 1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | MSH2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
