Application
Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 μg/mL.Application
Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 μg/mL.Application
Western blot analysis of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 μg /ml.| Product Name | MTBP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MTBP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.1) Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7. |
| Immunogen | MTBP antibody was raised against a 18 amino acid peptide near the center of human MTBP. The immunogen is located within amino acids 110 - 160 of MTBP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| MTBP antibody can be used for detection of MTBP by Western blot at 1 μg/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTBP |
|---|---|
| Alternative Names | MTBP Antibody: MDM2BP Mdm2-binding protein hMTBP |
| Molecular Weight(MW) | 104 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 μg/mL.
Application
Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 μg/mL.
Application
Western blot analysis of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 μg /ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | MTBP antibody can be used for detection of MTBP by Western blot at 1 μg/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MTBP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MTBP Antibody is supplied in PBS containing 0.02% sodium azide. |
