Application
Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.Application
Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.Application
Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | MTERFD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.1) Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79. |
| Immunogen | MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1 . The immunogen is located within the last 50 amino acids of MTERFD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTERFD1 |
|---|---|
| Alternative Names | MTERFD1 Antibody: CGI-12 mTERF3 mTERF domain-containing protein 1 mitochondrial Mitochondrial transcription termination factor 3 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 42 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members. |
Application
Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.
Application
Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.
Application
Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MTERFD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
