Application
Western blot analysis of lysate from SK-BR-3 cell line, using MTIF2 Antibody at 1:1000 at each lane.| Product Name | MTIF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2(mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2(mt) shares 32 to 38% amino acid sequence identity with yeast IF-2(mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].1) Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. (2010) In press : |
| Immunogen | This MTIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human MTIF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTIF2 |
|---|---|
| Alternative Names | Translation initiation factor IF-2 mitochondrial IF-2(Mt) IF-2Mt IF2(mt) MTIF2 |
| Molecular Weight(MW) | 81 kDa |
Application
Western blot analysis of lysate from SK-BR-3 cell line, using MTIF2 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
