Application
Western blot showing knockdown of endogenous MTM1 expression by MTM1-targeting vectors pDM134 and pDM170. Embryonic stem (ES) cells were untreated (lane 1) or transfected with control plasmid pDCont (lane 2), MTM1-targeting plasmid pDM134 (lane 3), or pDM170 (lane 4). The blot was probed with anti-MTM1 rabbit polyclonal antibodies. -Actin was used as a loading control.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | MTM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MTM1 is a member of a protein family that encodes tyrosine phosphatases. Myotubularin is required for muscle cell differentiation and mutations in MTM1 have been identified as being responsible for X-linked myotubular myopathy. MTM1 is a potent phosphatidylinositol 3-phosphate phosphatase (PI(3)P). Mutations in the MTM1 gene that cause human myotubular myopathy dramatically reduce the ability of the phosphatase to dephosphorylate PI(3)P. The findings provided evidence that myotubularin exerts its effects during myogenesis by regulating the cellular levels of the inositol lipid PI(3)P.1) Nandurkar, H.H., et al., Proc. Natl. Acad. Sci. U.S.A. 100(15):8660-8665 (2003). |
| Immunogen | This MTM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 566-594 amino acids from the C-terminal region of human MTM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MTM1 |
|---|---|
| Alternative Names | Myotubularin Phosphatidylinositol-3 5-bisphosphate 3-phosphatase Phosphatidylinositol-3-phosphate phosphatase MTM1 CG2 |
| Molecular Weight(MW) | 70 kDa |
Application
Western blot showing knockdown of endogenous MTM1 expression by MTM1-targeting vectors pDM134 and pDM170. Embryonic stem (ES) cells were untreated (lane 1) or transfected with control plasmid pDCont (lane 2), MTM1-targeting plasmid pDM134 (lane 3), or pDM170 (lane 4). The blot was probed with anti-MTM1 rabbit polyclonal antibodies. -Actin was used as a loading control.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
