Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Product Name | Munc-18 (phospho Ser515) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Munc-18 ( mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).1) Ciufo LF, Barclay JW, Burgoyne RD, Morgan A (2005) Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions. Mol Biol Cell 16:470-482. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser515 of Munc-18. |
| Modification | p-Ser515 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. | |
| Species Reactivity | BovineChickenDogMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Stxbp2 |
|---|---|
| Alternative Names | Munc18b Unc18b Protein unc-18 homolog 2 Munc18-2 |
| Molecular Weight(MW) | 65 |
Application
Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. |
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| Form | Liquid |
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| Storage Instructions | Munc-18 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
