Application
Western blot analysis of extracts from JK cells (Lane 2), using MuSK (Phospho-Tyr755) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | MuSK (phospho Tyr755) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane.1) Valenzuela D.M., Neuron 15:573-584(1995). |
| Immunogen | MuSK (phospho Tyr755) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 755 (A-D-Y (p)-Y-K) derived from Human MuSK . |
| Modification | p-Tyr755 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MUSK |
|---|---|
| Alternative Names | MUSK Muscle skeletal |
| Molecular Weight(MW) | 97 kDa |
| Tissue Specificity | The antibody detects endogenous levels of MuSK only when phosphorylated at tyrosine 755. |
| Entrez Gene | 4593 |
|---|---|
| Protein Accession | NP_005583.1 |
Application
Western blot analysis of extracts from JK cells (Lane 2), using MuSK (Phospho-Tyr755) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
