Application
Confocal immunofluorescent analysis of MUTYH Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of MUTYH Antibody in mouse lung tissue lysates (35ug/lane)Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MUTYH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches. MYH is involved in oxidative DNA damage repair. It initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. It possesses both adenine and 2-OH-A DNA glycosylase activities.1) Kim C.J., et.al., Oncogene 23:6820-6822(2004). |
| Immunogen | This MUTYH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-367 amino acids from the Central region of human MUTYH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | MUTYH |
|---|---|
| Alternative Names | A/G-specific adenine DNA glycosylase 322- MutY homolog hMYH MUTYH MYH |
| Molecular Weight(MW) | 60 kDa |
Application
Confocal immunofluorescent analysis of MUTYH Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of MUTYH Antibody in mouse lung tissue lysates (35ug/lane)
Application
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
