Application
Immunofluorescence of MYBPC1 in mouse skeletal muscle cells with MYBPC1 antibody at 20 μg/mL.Application
Western blot analysis of MYBPC1 in rat skeletal muscle tissue lysate with MYBPC1 antibody at 1 μg/mL.| Product Name | MYBPC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYBPC1 Antibody: Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the slow-type isoform designated MYBPC1. Both the fast-type (MYBPC2) and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. Multiple isoforms of MYBPC1 are known to exist, and are present in varying amounts in different skeletal muscles. It is thought that the MYBPC1 slow subfamily may play important roles in the assembly and stabilization of sarcomeric M- and A-bands and regulate the contractile properties of the actomyosin filaments.1) Furst DO, Vinkemeir U and Weber K. Mammalian skeletal muscle C-protein: purification from bovine muscle, binding to titin and the characterization of a full-length human cDNA. J. Cell Sci. 1992; 102:769-78. |
| Immunogen | MYBPC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human MYBPC1. The immunogen is located within amino acids 160 - 210 of MYBPC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| MYBPC1 antibody can be used for detection of MYBPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYBPC1 |
|---|---|
| Alternative Names | MYBPC1 Antibody: LCCS4 MYBPCC MYBPCS Myosin-binding protein C slow-type C-protein skeletal muscle slow isoform Slow MyBP-C |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (73%) |
| Tissue Specificity | At least five isoforms of MYBPC1 are known to exist. MYBPC1 antibody is predicted not to cross-react with other MYBPC family members |
Application
Immunofluorescence of MYBPC1 in mouse skeletal muscle cells with MYBPC1 antibody at 20 μg/mL.
Application
Western blot analysis of MYBPC1 in rat skeletal muscle tissue lysate with MYBPC1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | MYBPC1 antibody can be used for detection of MYBPC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MYBPC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MYBPC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
