Application
Western blot analysis of MYBPC2 in mouse skeletal muscle tissue lysate with MYBPC2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of MYBPC2 in mouse skeletal muscle tissue with MYBPC2 antibody at 5 μg/mL.| Product Name | MYBPC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYBPC2 Antibody: Myosin binding protein C (MYBPC) is a component of the thick filament of striated muscle, with the fast-type isoform designated MYBPC2. Both the fast-type and slow-type MYBPC protein contains seven immunoglobulin C2 motifs and three fibronectin type-III repeats. MYBPC2 is typically required for strong contractions and functions under anaerobic conditions. It is more similar to the cardiac isoform (MYBPC3) than to the slow-type isoform (MYBPC1) in terms of pCa50-indexed force development, length-independent cooperativity and length dependent activation. It has been suggested that in cardiac and fast muscle MYBPC2 contributes to an internal load, possibly by binding to actin via its N-terminal region.1) Weber FE, Vaughan KT, Reiach FC, et al. Complete sequence of human fast-type and slow-type muscle myosin-binding-protein C (MyBP-C). Differential expression, conserved domain structure and chromosome assignment. Eur. J. Biochem.1993; 216:661-9. |
| Immunogen | MYBPC2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MYBPC2. The immunogen is located within amino acids 1070 - 1120 of MYBPC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYBPC2 |
|---|---|
| Alternative Names | MYBPC2 Antibody: MYBPC MYBPCF Myosin-binding protein C fast-type C-protein skeletal muscle fast isoform Fast MyBP-C |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (91%) |
Application
Western blot analysis of MYBPC2 in mouse skeletal muscle tissue lysate with MYBPC2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of MYBPC2 in mouse skeletal muscle tissue with MYBPC2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
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| Application Notes | MYBPC2 antibody can be used for detection of MYBPC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
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| Storage Instructions | MYBPC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MYBPC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
