Application
Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody at 1:1000.Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of lysate from rat heart tissue lysate, using MYBPC3 Antibody at 1:1000.Application
Antibody staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Western Blot at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | MYBPC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.1) Millat, G., et al. Clin. Chim. Acta 411 (23-24), 1983-1991 (2010) : |
| Immunogen | This MYBPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYBPC3 |
|---|---|
| Alternative Names | Myosin-binding protein C cardiac-type Cardiac MyBP-C C-protein cardiac muscle isoform MYBPC3 |
| Molecular Weight(MW) | 141 kDa |
Application
Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody at 1:1000.
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: human liver lysate Lane 2: mouse heart lysate Lane 3: rat heart lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of lysate from rat heart tissue lysate, using MYBPC3 Antibody at 1:1000.
Application
Antibody staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Western Blot at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
