Application
Immunohistochemistry of MyD88 in human heart with MyD88 antibody at 2.5 μg/mL.Application
Western blot analysis of MyD88 in EL4 whole cell lysate with MyD88 antibody at 2 μg/mL.| Product Name | MyD88 Antibody [2E9C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.1) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5. |
| Immunogen | Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAIFIHC-PWB |
| MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYD88 |
|---|---|
| Alternative Names | MyD88 Antibody [2E9C2] : MYD88D |
| Sequence Similarities | 3) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunohistochemistry of MyD88 in human heart with MyD88 antibody at 2.5 μg/mL.
Application
Western blot analysis of MyD88 in EL4 whole cell lysate with MyD88 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MyD88 monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
