Application
Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.Application
Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.| Product Name | MYH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).1) Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030. |
| Immunogen | MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3. The immunogen is located within amino acids 30 - 80 of MYH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH3 |
|---|---|
| Alternative Names | Myosin heavy chain 3 Myosin-3 Myosin heavy chain skeletal muscle embryonic HEMHC MYHC-EMB MYHSE1 SMHCE |
| Molecular Weight(MW) | Predicted: 240 kDa Observed: 245 kDa |
| Tissue Specificity | MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family. |
Application
Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.
Application
Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Human Skeletal Muscle Lysate |
|---|---|
| Application Notes | MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MYH3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MYH3 antibody is supplied in PBS containing 0.02% sodium azide. |
