Application
Immunohistochemistry of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 5 μg/ml.Application
Immunofluorescence of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 20 μg/ml.Application
Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.| Product Name | MYH8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).1) Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030. |
| Immunogen | MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8. The immunogen is located within amino acids 60 - 110 of MYH8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH8 |
|---|---|
| Alternative Names | Myosin heavy chain 8 Myosin-8 Myosin heavy chain skeletal muscle perinatal MyHC-perinatal MyHC-peri MyHC-pn gtMHC-F |
| Molecular Weight(MW) | Predicted: 213 kDa Observed: 220 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Horse: (95%) |
| Tissue Specificity | MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family. |
Application
Immunohistochemistry of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 5 μg/ml.
Application
Immunofluorescence of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 20 μg/ml.
Application
Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MYH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | MYH8 antibody is supplied in PBS containing 0.02% sodium azide. |
