Application
Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of MyoGEF Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | MyoGEF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG. Its part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, It is required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, it is required for normal macropinocytosis.1) D'Angelo,R., et.al., Mol. Biol. Cell 18 (12), 4780-4793 (2007) |
| Immunogen | This MyoGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-87 amino acids from the N-terminal region of human MyoGEF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLEKHG6 |
|---|---|
| Alternative Names | Pleckstrin homology domain-containing family G member 6 PH domain-containing family G member 6 Myosin-interacting guanine nucleotide exchange factor MyoGEF PLEKHG6 |
| Molecular Weight(MW) | 89 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of MyoGEF Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
