Application
Immunofluorescence staining of methanol-fixed HeLa cells using Myosin Light Chain 2 (Ab-18).Application
Western blot analysis of lysed extracts from K562 cells using Myosin Light Chain 2 (Ab-18).| Product Name | Myosin Light Chain 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion1) Janiak A, et al. (2006) Mol Biol Cell. Apr; 17 (4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26 (12): 4612-4627Li Z, et al. (2006) Mol Cell Biol. Jun; 26 (11): 4240-4256 |
| Immunogen | Myosin Light Chain 2 (Ab-18) antibody was raised against a peptide sequence around aa.16~20 (A-T-S-N-V) derived from Human Myosin Light Chain 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYL12A |
|---|---|
| Alternative Names | MLCB MRCL3 MRLC3 MYL2B HEL-S-24 MLC-2a MLC2a MLRA MYL7 MYLC2A |
| Molecular Weight(MW) | 18 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Myosin Light Chain 2 protein. |
| Entrez Gene | 10627 |
|---|---|
| Protein Accession | NP_006462.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Myosin Light Chain 2 (Ab-18).
Application
Western blot analysis of lysed extracts from K562 cells using Myosin Light Chain 2 (Ab-18).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
