Application
Immunohistochemistry of MYOZAP in human lung tissue with MYOZAP antibody at 5 μg/mL.Application
Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of MYOZAP in human lung tissue with MYOZAP antibody at 20 μg/mL.| Product Name | MYOZAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MYOZAP Antibody: MYOZAP, also known as GRINL1A, is a 54 kDa highly conserved cardiac protein. It is strongly expressed in the heart and lung and is a novel component of intercalated disc. MYOZAP interacts with myosin phosphatase-RhoA interacting protein (MRIP) and acts as an activator of Rho-dependent SRF signaling. Knockdown study in zebrafish results in cardiomyopathy with severe dysfunction. The MYOZAP gene is part of a complex transcript unit that includes the gene for glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (GRINL1A). Transcription of this gene occurs at an upstream promoter, with two different groups of alternatively spliced variants: Gup for GRINL1A upstream transcripts and Gcom for GRINL1A combined transcripts.1) Van Tintelen JP, Hofstra RM, Wiesfeld AC, et al. Molecular genetics of arrhythmogenic right ventricular cardiomyopathy: emerging horizon? Curr. Opin. Cardiol. 2007; 22:185-92. |
| Immunogen | MYOZAP antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human MYOZAP. The immunogen is located within amino acids 400 - 450 of MYOZAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GCOM1 |
|---|---|
| Alternative Names | MYOZAP Antibody: gcom Gcom2 MYZAP GRINL1A MYZAP-POLR2M MYOZAP Myocardial zonula adherens protein GRINL1A upstream protein Gup |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | Multiple isoforms of MYOZAP are known to exist. |
| Entrez Gene | 145781 |
|---|---|
| Protein Accession | NP_001018110 |
Application
Immunohistochemistry of MYOZAP in human lung tissue with MYOZAP antibody at 5 μg/mL.
Application
Western blot analysis of MYOZAP in rat kidney tissue lysate with MYOZAP antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of MYOZAP in human lung tissue with MYOZAP antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1465 - Rat Kidney Tissue Lysate |
|---|---|
| Application Notes | MYOZAP antibody can be used for detection of MYOZAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | MYOZAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | MYOZAP Antibody is supplied in PBS containing 0.02% sodium azide. |
