Application
Western Blot at 1:2000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HCT116 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NAA10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development.1) Tribioli C.,et al.Hum. Mol. Genet. 3:1061-1068(1994). |
| Immunogen | This NAA10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 135-167 amino acids from the Central region of human NAA10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NAA10 |
|---|---|
| Alternative Names | N-alpha-acetyltransferase 10 2.3.1.- 2.3.1.88 N-terminal acetyltransferase complex ARD1 subunit homolog A NatA catalytic subunit Naa10 NAA10 ARD1 ARD1A TE2 |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: SK-BR-3 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HCT116 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
