Application
Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.Application
Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide.| Product Name | NADE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NADE Antibody: The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.1) Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39. |
| Immunogen | NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| NADE antibody can be used for detection of NADE by Western blot at 1 μg/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NGFRAP1 |
|---|---|
| Alternative Names | NADE Antibody: Bex BEX3 NADE HGR74 DXS6984E Protein BEX3 Brain-expressed X-linked protein 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.
Application
Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | NADE antibody can be used for detection of NADE by Western blot at 1 μg/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NADE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NADE Antibody is supplied in PBS containing 0.02% sodium azide. |
