Application
Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 μg/mL.Application
Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 μg/mL.Application
Western blot analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 μg/mL.| Product Name | NAIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAIP Antibody: Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.1) Roy N, Mahadevan MS, McLean M, et al. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Cell 1995; 80:167-78. |
| Immunogen | NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP. The immunogen is located within amino acids 1200 - 1250 of NAIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NAIP |
|---|---|
| Alternative Names | NAIP Antibody: BIRC1 NLRB1 psiNAIP BIRC1 Baculoviral IAP repeat-containing protein 1 Neuronal apoptosis inhibitory protein |
| Molecular Weight(MW) | Predicted: 137, 154 kDa Observed: 155 kDa |
| Sequence Similarities | 3) Cat. No. 17-003 - A549 Cell Slide |
| Tissue Specificity | Minor, lower molecular weight bands may represent alternately spliced forms. |
Application
Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 μg/mL.
Application
Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 μg/mL.
Application
Western blot analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1315 - Human Tonsil Tissue Lysate |
|---|---|
| Application Notes | NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NAIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NAIP Antibody is supplied in PBS containing 0.02% sodium azide. |
