Application
Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.Application
Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 μg/mL.Application
Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 μg/mL.| Product Name | NALP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NALP1 Antibody: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.1) NALP1 antibody can be stored at 4˚C, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104. |
| Immunogen | NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1. The immunogen is located within the last 50 amino acids of NALP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRP1 |
|---|---|
| Alternative Names | NALP1 Antibody: NAC CARD7 CIDED NALP1 SLEV1 DEFCAP PP1044 VAMAS1 CLR17.1 DEFCAP-L/S KIAA0926 NAC NACHT LRR and PYD domains-containing protein 1 Caspase recruitment domain-containing protein 7 |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Application
Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 μg/mL.
Application
Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1215 - U937 Cell Lysate |
|---|---|
| Application Notes | NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NALP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NALP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
